Spectrophotometer Liquid

What is the difference between a spectroscope and a spectrophotometer?

I'm thinking about doing a science fair project concerning if different proteins dissolve at different rates in liquid, and I need some kind of instrument to measure the rate of dissolving of the proteins. I have done research upon this, and I have found about using a spectrophotometer. However, soon after that, when I was looking through some science catalogues, I also found spectroscopes along with spectrophotometers.

So... What if the difference between a spectroscope and a spectrophotometer? Also, which one should I use?

In addition, if there is any other kind of instrument that will help me accomplish this task, please let me know...

A spectroscope is a simple device, usually with a prism, which allows you to see spectral lines in light, with your own eyes, coming from a source, such as a star.

The spectrophotometer is much more sophisticated, using a light (ir, vis, uv) to project through anything translucent (solution, crystal, etc.) and a dectector coupled to a chart or computer so that a scan over a spectrum of wavelengths is displayed on one axis and the absorbance or transmittance on the other.

Spectrophotometers are expensive ($10K+). You might consider the following instrument:

A peice of polaroid film (nothing to do with the camera, same name as the corporation, founded by the great Edwin Land)
This film is the same as polarized sunglasses coating. It admits light in one plane, but not the plane perpendicular. For example, admits vertical, but blocks horizontal.

This film can be rotated in a circle in front of an illuminated cell containing your protein solution and, when it is clear or black, describes the optical rotation properties of the substances in the solution. These devices are widely used to gauge the concentration of sugars, as sugars rotate the plane of polarized light. Very, very cheap instrument. You can build yourself, very easily. Actually, that is a science fair project in itself. So, if you are in this to win, this is a great way to go. If your motive is to just participate, then the polarimeter, measuring the concentrations of some sugar solutions will suffice bueatifully. Also will teach you much more about physics and chemistry than just copping out by using a fancy gadget.

Study web articles on Louis Pasteur, Emil Fischer, Edwin Land, optical rotation, polarized light, polarimetery, polaroid film. You will have to familiarize yourself with polarized light and stereoisomers, but thats the fun! Best of luck!

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LaMotte SMART Spectro Spectrophotometer, 110V $1914 Features of the LaMotte SMART Spectro Spectrophotometer: A portable spectrophotometer that is easier to use and more accurate than anything in its price range. Over 80 pre-programmed tests included, and 25 user calibrations can be entered into the memory. The user can also customize sequences for frequently run tests. A wider wavelength range (350-1000 nm) Menu-driven display Pre-programmed tests with 25 user tests Automatic wavelength selection Unique optical design system using a 1200 lines/mm grating Greater accuracy, higher resolution The SMART Spectro is supplied with 6 sample tubes (25mm round), 2 sample cell holders (25mm round and COD, 10 mm cuvettes), AC adapter, battery charger, instruction manual including test procedures, and quick start guides.

Shimadzu UV-Mini 1240 Spectrophotometer $5791 Features of the Shimadzu UV-Mini 1240 Spectrophotometer: Features of a full-scale spectrophotometer in a benchtop unit. Simple enough for the general lab user to use with only minimal training. Functions range from simple concentration measurements to complex calibration curves. Large LCD displays prompts, keypad-function descriptions, and graphics. Multipoint calibration curve for applications that require various standards. K-factor, as well as first-, second-, or third-order polynomial fits. Two- or three-wavelength quantitative analysis for measuring turbid samples or the effects of a distinguishable component. Peak-pick function detects even the most sensitive wavelengths. Repeat scan automatically reveals spectral change over the entire range. Photometric mode can be used to measure absorbance or transmittance at fixed wavelengths. Quantification mode can be used to determine concentrations of unknown samples in single-component analyses. Methods, results, and even raw data can be saved on the unit or to a PC with optional software. Print results with ease, choosing optional color inkjet printer and software. Includes: Single cell holder

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Labomed Spectro UV-VIS Double Beam Research Spectrophotometer $11304 Features of the Labomed Spectro UV-VIS Double Beam Research Spectrophotometer: Spectro UV-VIS Double Beam UVD 3500 Research Spectrophotometer is a superior instrument for the research laboratory and is an advanced and affordable system that generates accurate and reproducible measurements. UVD-3500 spectrophotometer is accurate, reliable, and an exceptional value. With its narrow beam design, the system provides optimal and reproducible results for micro and macro samples with high resolution for protein, nucleic acid, and DNA/RNA analysis.Spectro UV-VIS Double Beam UVD 3500 has a powerful built in software which permits this instrument to be linked to a computer and a printer to display the photometric and spectral data on the PC monitor. This spectrophotometer is rugged, reliable, affordable, and maintenance free. Spectro UV-VIS Double Beam UVD 3500’s enhanced transmission and full reflection makes this double beam spectrophotometer highly effective and reduces noise. Spectro UV-VIS Double Beam UVD 3500’s advantage is its accurate wavelength, ease of operation, versatile software application, and effortless optional accessory installation. This instrument can be used for analyzing solid samples through use of an optional reflectance accessory and integrating sphere. Wavelength: 190-900nm Double beam auto scanning Variable bandwidth: 0.1, 0.2, 0.5, 1.0, 2.0, 5.0nm Micro/macro sample vol. w/high absorbance

Unico SpectroQuest UV-Visible SQ2802A Spectrophotometer $6211.77 Features of the Unico SpectroQuest UV-Visible SQ2802A Spectrophotometer: Wavelength Range: 190 - 1100 nm. Spectral Bandwidth: 1.8 nm / 0.5, 1, 2, 5 nm. Monochromator: Single beam Littrow type, grating 1200 lines/mm. Wavelength Accuracy: ±0.3 nm. Wavelength Repeatability: ±0.2 nm. Wavelength Calibration: automatic at start. Light Source: Tungsten Halogen/Deuterium Lamp. Detector: Solid Silicon Photodiode. Photometric Readout: 1/4 VGA 320X240 pixels backlit LCD. Photometric Range: 0 - 200%T -0.3 - 3.0A 0 - 9999C. Photometric Accuracy: ±0.3%T. Zero Drift: < 0.002A per hour after warm-up. Baseline Flatness: ±0.004A. Stability: 0.002A/h at 500 nm. Stray Light: < 0.15%T. Scanning Speed: Hi, Med., Low. Max. 1000 nm/min. Standard Sample Holder: 8-position Cell Holder. Analogue/Digital Output: Digital, RS232 Serial, Centronics Parallel. Dimensions: 620(L) x 400(W) x 280(H) mm. Weight: 22 kg / 48 lbs. Shipping Weight: 26 kg / 57 lbs. Power Requirements: 115V/60Hz or 230V/50Hz, switchable. Humidity Range: less than 85%. Safety Certificate: CE.

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Biology people help me!?

Let’s say a reaction between an enzyme and a substrate occurs. 3 trials were done, with the purpose of determining the reaction rates by looking at change in absorbance using a spectrophotometer, and according to a graph all three reaction rates were vastly different. Yet all three trials contained the same temperature, pH, and ionic concentration levels. The same amount of substrate and enzyme were used for all three tests too. But the only thing that seems to have caused this change the chart says that there are 6mL of “other liquids”. What possible explanations are there for these results?

This was a lab analysis question. When you answer this question consider the “other liquids” as the solution last. I know that the other liquids maybe contain inhibitors but I’m afraid I might have missed something while I was thinking it through.

That’s about all the info that is given in the question. (the “other liquids” are not specified)

The other liquids may contain inhibitors, effectors (something that speeds up the reaction), and/or combinations of both.

For example, the fastest reaction rate may have contained only effectors in the "other liquids".

The slowest reaction rate may have contained only inhibitors.

And, the intermediate rate might have contained both effectors and inhibitors - or, neither!

Best wishes and good luck.