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Limited Photomultiplier

What factors define the upper and lower detection limits of a photomultiplier?
In atomic absorption spectroscopy, the photomultiplier detector is what obviously tells us the wavelength and hence type of metal we are analysing, but I'm not fully aware of the detector's response to photons in different situations. I'd just like to know a few of the factors/conditions under which the detector provides an alternative response ie its upper and lower detection limits.
Lower detection limit is the noise current of the system. If you crank the voltage up inside the tube too much you will start to pull electrons off the surface of the last dynode -- even though there are no photon-induced electrons hitting it. That is a false reading.
The high detection limit is when the dynodes get saturated and no more electrons can be knocked off because they are already all 'gone' (they cannot be replaced fast enough via the bias current power supply). Now if more photons are coming in the front end, they will not register as a corresponding rise in current. The current will plateau and not get any higher.
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Cardiovascular Innovations Yibao Wu
Chemiluminescence
Chemiluminescence which is also known as the "chemo luminescence" the light emission featuring limited emission of heat (luminescence), as the result of a chemical reaction. As formulated by a theory, a photon of light should be released for each molecule of reactant, or Avogadro's number of photons per mole. In actual situation, the non-enzymatic reactions rarely surpass 1% QC, quantum efficiency.
This can be found in the luminal test, where evidence of blood is taken when the sample when mixed with iron. When the reaction takes place in living organisms, the event is called bioluminescence. A light stick releases a form of light by chemiluminescence. This is the normal example of chemiluminescence in the laboratory setting. One of its oldest known reactions is the oxidation of elemental white phosphorus in most air for the production of green glow. Thus is the exact gas-stage reaction of phosphorus vapor, featuring an oxygen that generates the excited states. Another gas phase is based from the nitric oxide detection in commercial analytic instruments which is quite useful to environmental air quality testing. Ozone is joined with nitric oxide to create nitrogen dioxide in an activated state.
The stimulated NO2 luminance broadband is seen to infrared light as it rotates to a lower energy state. A photomultiplier and associated electronics that calculates the photons are relative to the amount of NO present. If there is a need to identify the amount of nitrogen dioxide in a sample (containing a NO) it must first be transformed to nitric oxide. This is made possible by passing the sample through a converter before the application of the above ozone activation reaction. The ozone reaction creates a photon count relative to NO which is proportional to NO2 before it was actually transformed to NO. If the mixed sample has both NO and NO2, the above reaction gives way to the amount of NO and NO2 combined in the air sample.
About the Author
Dominique Rutherford


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